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Addgene

p2129
(Plasmid #198143)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198143 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWSK29
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PsseJ sseJ::M45
  • Species
    Salmonella enterica serovar Typhimurium
  • Mutation
    P290S mutation in sseJ
  • Entrez Gene
    sseJ (a.k.a. STM1631)
  • Tag / Fusion Protein
    • M45 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

P290S mutation in sseJ insert does not affect protein translocation or subcellular targeting.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2129 was a gift from Michael Hensel (Addgene plasmid # 198143 ; http://n2t.net/addgene:198143 ; RRID:Addgene_198143)
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