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Addgene

pCI-Puro_hCOL4A4-Myc-His
(Plasmid #198093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COL4A4
  • Species
    H. sapiens (human)
  • Entrez Gene
    COL4A4 (a.k.a. ATS2, BFH, BFH1, CA44)
  • Promoter CMV, beta-globin/IgG chimeric intron
  • Tag / Fusion Protein
    • TEV-Myc-His (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Puro_hCOL4A4-Myc-His was a gift from Jeffrey Miner (Addgene plasmid # 198093 ; http://n2t.net/addgene:198093 ; RRID:Addgene_198093)
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