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Addgene

pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry
(Plasmid #198069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4425
  • Total vector size (bp) 6162
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iRFP713(C15S/V256C)
  • Species
    Synthetic
  • Insert Size (bp)
    1737
  • Mutation
    C15S/V256C mutation
  • Promoter CAG
  • Tag / Fusion Protein
    • P2A-mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTAGAGCCTCTGCTAACCATGTTCATGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Vladislav Verkhusha (Addgene plasmid # 45468: pNLS-iRFP713)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry was a gift from Michiyuki Matsuda (Addgene plasmid # 198069 ; http://n2t.net/addgene:198069 ; RRID:Addgene_198069)
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