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Addgene

pCSIIbleo-miRFP670nano-P2A-EGFP
(Plasmid #198064)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSIIbleo
  • Backbone size w/o insert (bp) 10120
  • Total vector size (bp) 11326
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miRFP670nano
  • Species
    Synthetic
  • Insert Size (bp)
    1206
  • Promoter CAG
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TCATTCTCAAGCCTCAGACAGTGG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Vladislav Verkhusha (Addgene plasmid # 127443: miRFP670nano-pcDNA)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSIIbleo-miRFP670nano-P2A-EGFP was a gift from Michiyuki Matsuda (Addgene plasmid # 198064 ; http://n2t.net/addgene:198064 ; RRID:Addgene_198064)
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