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pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE
(Plasmid #198054)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198054 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJT126
  • Modifications to backbone
    Used assembly PCR to construct a cassette with a TRE3G promoter followed by a 3xFLAG tag, a Golden Gate library cloning site, an SV40 polyA tail, and the core sequence of the chicken HS4 insulator; used Gibson to clone in between the NotI and NsiI restriction sites. Used assembly PCR and Gibson cloning to replace the rTetR(SE-G72P)-3xFLAG-LibCloneSite with rTetR(SEG72P)-VP48.
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
  • Species
    Synthetic
  • Insert Size (bp)
    1929
  • Promoter pEF

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    3xFLAG-insert
  • Species
    Synthetic
  • Insert Size (bp)
    117
  • Promoter TRE3G
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE was a gift from Lacra Bintu (Addgene plasmid # 198054 ; http://n2t.net/addgene:198054 ; RRID:Addgene_198054)

  • For your References section:

    High-throughput discovery and characterization of viral transcriptional effectors in human cells. Ludwig CH, Thurm AR, Morgens DW, Yang KJ, Tycko J, Bassik MC, Glaunsinger BA, Bintu L. Cell Syst. 2023 Jun 21;14(6):482-500.e8. doi: 10.1016/j.cels.2023.05.008. 10.1016/j.cels.2023.05.008 PubMed 37348463
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