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pL1P6 ZmUbiP:GRF-GIF:NosT
(Plasmid #198049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH47781 AddGene #48005
  • Backbone manufacturer
    Sylvestre Marillonnet
  • Backbone size w/o insert (bp) 4348
  • Total vector size (bp) 8543
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZmUbiP::TaGRF4-GIF1::NosT
  • Alt name
    Overexpression Growth-Regulating Factor 4 and cofactor GRF-Interacting Factor 1
  • Species
    Synthetic
  • Promoter Zea mays (maize) ubiquitin promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco31I (BsaI) (destroyed during cloning)
  • 3′ cloning site Eco31I (BsaI) (destroyed during cloning)
  • 5′ sequencing primer GAACCCTGTGGTTGGCATGCACATAC
  • 3′ sequencing primer CTGGTGGCAGGATATATTGTGGTG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol 38, 1274–1279 (2020). We acknowledge the researchers at UC Davis and HHMI for the development of GRF4-GIF1 fusion reported in PMID: 33046875.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL1P6 ZmUbiP:GRF-GIF:NosT was a gift from John Innes Centre - Crop Transformation and Genome Editing Group & Cristobal Uauy (Addgene plasmid # 198049 ; http://n2t.net/addgene:198049 ; RRID:Addgene_198049)
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