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Addgene

pRD323
(Plasmid #198044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Backbone size w/o insert (bp) 5204
  • Total vector size (bp) 5414
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HMfA K31A E35A
  • Species
    Synthetic
  • Insert Size (bp)
    210
  • Promoter T7 promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer cgtttagaggccccaaggggttatgctag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRD323 was a gift from Remus Dame (Addgene plasmid # 198044 ; http://n2t.net/addgene:198044 ; RRID:Addgene_198044)

  • For your References section:

    Specific DNA binding of archaeal histones HMfA and HMfB. Erkelens AM, Henneman B, van der Valk RA, Kirolos NCS, Dame RT. Front Microbiol. 2023 Apr 18;14:1166608. doi: 10.3389/fmicb.2023.1166608. eCollection 2023. 10.3389/fmicb.2023.1166608 PubMed 37143534
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