pBR_attB(bxb)_ccdB_lox_v2
(Plasmid
#198040)
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Purpose(Empty Backbone) Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 198040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
- Backbone size (bp) 5200
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Vector typeMammalian Expression, Cre/Lox, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsPresence of ccdB gene
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBR_attB(bxb)_ccdB_lox_v2 was a gift from Richard Davis (Addgene plasmid # 198040 ; http://n2t.net/addgene:198040 ; RRID:Addgene_198040) -
For your References section:
STRAIGHT-IN: a platform for rapidly generating panels of genetically modified human pluripotent stem cell lines. Blanch-Asensio A, Grandela C, Mummery CL, Davis RP. Nat Protoc. 2024 Aug 23. doi: 10.1038/s41596-024-01039-2. 10.1038/s41596-024-01039-2 PubMed 39179886
Map uploaded by the depositor.
