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pCAGG-GST-TEV-mCherry-NAP1 (WT)
(Plasmid #198036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGG
  • Backbone size w/o insert (bp) 4790
  • Total vector size (bp) 7445
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NAP1
  • Alt name
    AZI2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2595
  • GenBank ID
    AY151386
  • Entrez Gene
    AZI2 (a.k.a. AZ2, NAP1, TILP)
  • Tags / Fusion Proteins
    • GST-TEV (N terminal on insert)
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ccaccATgtcccctatactaggttattggaaaattaagggcc
  • 3′ sequencing primer attcttataaaggcagttctgatt
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGG-GST-TEV-mCherry-NAP1 (WT) was a gift from Sascha Martens (Addgene plasmid # 198036 ; http://n2t.net/addgene:198036 ; RRID:Addgene_198036)

  • For your References section:

    Unconventional initiation of PINK1/Parkin mitophagy by Optineurin. Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M. Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. 10.1016/j.molcel.2023.04.021 PubMed 37207627
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