pSH124-ssr
(Plasmid #198025)

• Purpose: Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E
• Depositing Lab: Susanne Häußler
• Publication: Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41596-023-00882-z.

Backbone

• Vector backbone: pSH624-ssr
• Backbone size w/o insert (bp): 4948
• Total vector size (bp): 5888
• Vector type: Bacterial Expression
• Selectable markers: Carbenicillin 0.4 mg/ml for P. aeruginosa

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: T0 terminator - Ap/Cb resistance cassette
• Species: Synthetic
• Insert Size (bp): 1175
• GenBank ID:
• Promoter: ApR promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BcuI (not destroyed)
• 3′ cloning site: BoxI (not destroyed)
• 5′ sequencing primer: SEVA_T0-F
• 3′ sequencing primer: PS4

Resource Information

• Supplemental Documents:
  • pSH124-ssr.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry