pCCL-c-MNDU3-CD3D-274+5G>A
(Plasmid
#198023)
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PurposeLV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in Ecuador
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCCL-c-MNDU3
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD3D c.274+5G>A
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SpeciesH. sapiens (human)
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MutationCodon optimized (20 bp) on either end of cDNA for PCR amplification; CD3D c.274+5G>A
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCCL-c-MNDU3-CD3D-274+5G>A was a gift from Donald Kohn (Addgene plasmid # 198023 ; http://n2t.net/addgene:198023 ; RRID:Addgene_198023)