eGFP-Silicatein-pET28
(Plasmid #198012)

• Purpose: C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification
• Depositing Lab: Bryan Berger
• Publication: Vigil et al Materials Advances 2, 2023

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: eGFP-silicatein
• Species: Synthetic
• Insert Size (bp): 1500
• Promoter: T7
• Tags / Fusion Proteins:
  • hexahistidine tag (N terminal on backbone)
  • hexahistidine tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: gggtcgcggatccATGGT
• 3′ sequencing primer: CCCACCCTCGAGTTGATTGTATTTGTT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry