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Addgene

pLexM-SLC7A11_GFP
(Plasmid #197995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLexM
  • Backbone manufacturer
    Plasmid 99844
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC7A11
  • Alt name
    System Xc-
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1527
  • GenBank ID
    BAA82628.1 BAA82628.1
  • Entrez Gene
    SLC7A11 (a.k.a. CCBR1, xCT)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG tag (N terminal on insert)
    • TEV-GFP-His8 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CMV_F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLexM-SLC7A11_GFP was a gift from Simon Newstead (Addgene plasmid # 197995 ; http://n2t.net/addgene:197995 ; RRID:Addgene_197995)

  • For your References section:

    Molecular basis for redox control by the human cystine/glutamate antiporter system xc(). Parker JL, Deme JC, Kolokouris D, Kuteyi G, Biggin PC, Lea SM, Newstead S. Nat Commun. 2021 Dec 8;12(1):7147. doi: 10.1038/s41467-021-27414-1. 10.1038/s41467-021-27414-1 PubMed 34880232
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