Skip to main content
Addgene

pLexM-SLC3A2
(Plasmid #197994)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197994 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLexM
  • Backbone manufacturer
    Plasmid 99844
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC3A2
  • Alt name
    4f2hc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1731
  • Mutation
    Please see depositor comments below
  • GenBank ID
    J02939.1 J02939.1
  • Entrez Gene
    SLC3A2 (a.k.a. 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE)
  • Promoter CMV
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a P539S mutation in SLC3A2. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLexM-SLC3A2 was a gift from Simon Newstead (Addgene plasmid # 197994 ; http://n2t.net/addgene:197994 ; RRID:Addgene_197994)

  • For your References section:

    Molecular basis for redox control by the human cystine/glutamate antiporter system xc(). Parker JL, Deme JC, Kolokouris D, Kuteyi G, Biggin PC, Lea SM, Newstead S. Nat Commun. 2021 Dec 8;12(1):7147. doi: 10.1038/s41467-021-27414-1. 10.1038/s41467-021-27414-1 PubMed 34880232
Related items:
Commonly requested with: