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Addgene

pSB_syb25
(Plasmid #197992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB_init
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sybody 25
  • Species
    Synthetic
  • Insert Size (bp)
    441
  • Promoter Arabinose
  • Tag / Fusion Protein
    • Myc-His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspQ1 (not destroyed)
  • 3′ cloning site BspQ1 (not destroyed)
  • 5′ sequencing primer pBAD_F
  • 3′ sequencing primer pBAD_R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB_syb25 was a gift from Simon Newstead (Addgene plasmid # 197992 ; http://n2t.net/addgene:197992 ; RRID:Addgene_197992)

  • For your References section:

    Molecular basis for selective uptake and elimination of organic anions in the kidney by OAT1. Parker JL, Kato T, Kuteyi G, Sitsel O, Newstead S. Nat Struct Mol Biol. 2023 Jul 23. doi: 10.1038/s41594-023-01039-y. 10.1038/s41594-023-01039-y PubMed 37482561
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