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PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290
(Plasmid #197989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCDH-CMV-MCS-EF1a-gfp-T2A-puro
  • Backbone size w/o insert (bp) 7384
  • Total vector size (bp) 7588
  • Modifications to backbone
    For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 has been cloned into PCDH-CMV-MCS-EF1a-gfp-T2A-puro by EcoRI-BamHI cloning site.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSALNT0217290
  • Alt name
    HSALNG0104472
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    227
  • Tag / Fusion Protein
    • lac (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290 was a gift from Bo Wang (Addgene plasmid # 197989 ; http://n2t.net/addgene:197989 ; RRID:Addgene_197989)

  • For your References section:

    Copy number variation-associated lncRNAs may contribute to the etiologies of congenital heart disease. Lu Y, Fang Q, Qi M, Li X, Zhang X, Lin Y, Xiang Y, Fu Q, Wang B. Commun Biol. 2023 Feb 17;6(1):189. doi: 10.1038/s42003-023-04565-z. 10.1038/s42003-023-04565-z PubMed 36806749
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