pETDuet-MerTK-PTPN1
(Plasmid #197988)

• Purpose: Expression of MerTK Kinase Domain (wild-type) in E. coli
• Depositing Lab: Byung Il Lee
• Publication: Bae et al Exp Mol Med. 2022 Sep;54(9):1450-1460. doi: 10.1038/s12276-022-00840-x. Epub 2022 Sep 2.

Backbone

• Vector backbone: pETduet1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5420
• Modifications to backbone: Thrombin cleavage site was inserted deliberately
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Tyrosine-Protein Kinase Mer
• Alt name: MERTK
• Species: H. sapiens (human)
• Insert Size (bp): 888
• Entrez Gene: MERTK (a.k.a. MER, RP38, Tyro12, c-Eyk, c-mer)
• Promoter: T7 promoter

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pET upstream
• 3′ sequencing primer: DuetDOWN1

Gene/Insert 2

• Gene/Insert name: PTPN1
• Alt name: PTP1B
• Species: H. sapiens (human)
• Insert Size (bp): 990
• Entrez Gene: PTPN1 (a.k.a. PTP1B)
• Promoter: T7 promoter

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: Acc65I (not destroyed)
• 5′ sequencing primer: DuetUP2
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry