Skip to main content
Addgene

pMG81-MBP-t-M.MpeI-N374K-His
(Plasmid #197985)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMG81
  • Backbone manufacturer
    Mark Goulian
  • Backbone size w/o insert (bp) 3228
  • Total vector size (bp) 5640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ER1821
  • Growth instructions
    The CpG Carboxymethyltransferase encoded by this plasmid can only be propagated in strains such as NEB ER1821 which are deleted of methylation recognizing restriction factors.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MBP-t-M.MpeI-N374K-His
  • Species
    M. penetrans
  • Insert Size (bp)
    2412
  • Mutation
    M.MpeI-N374K
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer GACACTCTATCATTGATAG
  • 3′ sequencing primer CGTTCACCGACAAACAAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMG81-MBP-t-M.MpeI-N374K-His was a gift from Rahul Kohli (Addgene plasmid # 197985 ; http://n2t.net/addgene:197985 ; RRID:Addgene_197985)

  • For your References section:

    Direct enzymatic sequencing of 5-methylcytosine at single-base resolution. Wang T, Fowler JM, Liu L, Loo CE, Luo M, Schutsky EK, Berrios KN, DeNizio JE, Dvorak A, Downey N, Montermoso S, Pingul BY, Nasrallah M, Gosal WS, Wu H, Kohli RM. Nat Chem Biol. 2023 Jun 15. doi: 10.1038/s41589-023-01318-1. 10.1038/s41589-023-01318-1 PubMed 37322153
Related items:
Commonly requested with: