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Addgene

TRF2-IRESS-eGFP
(Plasmid #19798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19798 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TRF2
  • Alt name
    TERF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    When compared to GenBank reference sequence NP_005643.1, TRF2 insert is missing aa434
  • GenBank ID
    NM_005652.1 NP_005643.1
  • Entrez Gene
    TERF2 (a.k.a. TRBF2, TRF2)
  • Promoter CMV-F

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    de Lange
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRF2-IRESS-eGFP was a gift from Mark Mattson (Addgene plasmid # 19798 ; http://n2t.net/addgene:19798 ; RRID:Addgene_19798)

  • For your References section:

    TRF2 protects human telomeres from end-to-end fusions. van Steensel B, Smogorzewska A, de Lange T. Cell. 1998 Feb 6. 92(3):401-13. 10.1016/S0092-8674(00)80932-0 PubMed 9476899
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