Skip to main content
Addgene

pET-20b_HtrA2(delta(133))_S306A_6His
(Plasmid #197975)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-20b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HtrA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    984
  • Mutation
    delta(1-133), S306A
  • Entrez Gene
    HTRA2 (a.k.a. MGCA8, OMI, PARK13, PRSS25)
  • Tag / Fusion Protein
    • 6His (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Electroporation recommended.

Please visit https://www.biorxiv.org/content/10.1101/2022.08.19.504532v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-20b_HtrA2(delta(133))_S306A_6His was a gift from Eric Fischer (Addgene plasmid # 197975 ; http://n2t.net/addgene:197975 ; RRID:Addgene_197975)

  • For your References section:

    Structures of BIRC6-client complexes provide a mechanism of SMAC-mediated release of caspases. Hunkeler M, Jin CY, Fischer ES. Science. 2023 Mar 17;379(6637):1105-1111. doi: 10.1126/science.ade5750. Epub 2023 Feb 9. 10.1126/science.ade5750 PubMed 36758104
Related items: