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Addgene

pDONR223_BIRC6
(Plasmid #197974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BIRC6
  • Alt name
    BRUCE
  • Alt name
    APOLLON
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    14571
  • Mutation
    L1332V
  • Entrez Gene
    BIRC6 (a.k.a. APOLLON, BRUCE)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Electroporation recommended.

Please visit https://www.biorxiv.org/content/10.1101/2022.08.19.504532v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223_BIRC6 was a gift from Eric Fischer (Addgene plasmid # 197974 ; http://n2t.net/addgene:197974 ; RRID:Addgene_197974)

  • For your References section:

    Structures of BIRC6-client complexes provide a mechanism of SMAC-mediated release of caspases. Hunkeler M, Jin CY, Fischer ES. Science. 2023 Mar 17;379(6637):1105-1111. doi: 10.1126/science.ade5750. Epub 2023 Feb 9. 10.1126/science.ade5750 PubMed 36758104
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