TRF2ΔBΔM/pENTR
(Plasmid
#19797)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/D-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2820
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Vector typeEntry Vector
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRF2
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Alt nameTERF2
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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Mutationdeleted amino acids 1-44 and 450-500
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GenBank IDNM_005652.1 NP_005643.1
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Entrez GeneTERF2 (a.k.a. TRBF2, TRF2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pENTR4-R
- 3′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byde Lange
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The missing codon is due to a reverse primer with a stop codon at 1474 of TRF2 gene: GAATTC TTA CTTCTGCTTTTTTGTTATATTGGTTGT. It generates a 50aa in frame deletion from c-terminal tail.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRF2ΔBΔM/pENTR was a gift from Mark Mattson (Addgene plasmid # 19797 ; http://n2t.net/addgene:19797 ; RRID:Addgene_19797) -
For your References section:
TRF2 protects human telomeres from end-to-end fusions. van Steensel B, Smogorzewska A, de Lange T. Cell. 1998 Feb 6. 92(3):401-13. 10.1016/S0092-8674(00)80932-0 PubMed 9476899
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/60/26/73af409e-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)