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Addgene

TRF2ΔBΔM/pENTR
(Plasmid #19797)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2820
  • Vector type
    Entry Vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRF2
  • Alt name
    TERF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Mutation
    deleted amino acids 1-44 and 450-500
  • GenBank ID
    NM_005652.1 NP_005643.1
  • Entrez Gene
    TERF2 (a.k.a. TRBF2, TRF2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pENTR4-R
  • 3′ sequencing primer M13pUC-fwd
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    de Lange

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The missing codon is due to a reverse primer with a stop codon at 1474 of TRF2 gene: GAATTC TTA CTTCTGCTTTTTTGTTATATTGGTTGT. It generates a 50aa in frame deletion from c-terminal tail.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRF2ΔBΔM/pENTR was a gift from Mark Mattson (Addgene plasmid # 19797 ; http://n2t.net/addgene:19797 ; RRID:Addgene_19797)

  • For your References section:

    TRF2 protects human telomeres from end-to-end fusions. van Steensel B, Smogorzewska A, de Lange T. Cell. 1998 Feb 6. 92(3):401-13. 10.1016/S0092-8674(00)80932-0 PubMed 9476899
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