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pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
(Plasmid #197958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCambia1300
  • Total vector size (bp) 15013
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    pcoCasphi-2
  • Alt name
    Arabidopsis codon optimized Casphi-2
  • Alt name
    Cas12j2
  • Species
    Synthetic
  • Insert Size (bp)
    2460
  • Promoter pUBQ10

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGATTTCTATCTAGATCTGGTGTTAGT
  • 3′ sequencing primer CTGATGCATTGAACTTGACGAACGT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    AtPDS3 gRNA10
  • Alt name
    AtPDS3 guide RNA10 driven by the CmYLCV promoter and flanked by ribozymes
  • Alt name
    cagttgacaatccagccaat
  • Species
    Synthetic
  • Promoter CmYLCVp

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGGCAGACATACTGTCCCACA
  • 3′ sequencing primer ACGTTGTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10 was a gift from Steven Jacobsen (Addgene plasmid # 197958 ; http://n2t.net/addgene:197958 ; RRID:Addgene_197958)

  • For your References section:

    Genome editing in plants using the compact editor CasPhi. Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna JA, Jacobsen SE. Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 10.1073/pnas.2216822120. Epub 2023 Jan 18. 10.1073/pnas.2216822120 PubMed 36652483
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