pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10
(Plasmid #197952)

• Purpose: T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.
• Depositing Lab: Steven Jacobsen
• Publication: Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 10.1073/pnas.2216822120. Epub 2023 Jan 18.

Backbone

• Vector backbone: pCambia1300
• Total vector size (bp): 14769
• Vector type: Plant Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: pcoCasphi-2
• Alt name: Arabidopsis codon optimized Casphi-2
• Alt name: Cas12j2
• Species: Synthetic
• Insert Size (bp): 2460
• Promoter: pUBQ10

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGATTTCTATCTAGATCTGGTGTTAGT
• 3′ sequencing primer: CTGATGCATTGAACTTGACGAACGT

Gene/Insert 2

• Gene/Insert name: AtPDS3 gRNA10
• Alt name: AtPDS3 guide RNA10 driven by the AtU6-26 promoter
• Alt name: cagttgacaatccagccaat
• Species: Synthetic
• Promoter: AtU6-26

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTAGAAGCTTCGTTGAACAACGGA
• 3′ sequencing primer: ACGTTGTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry