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Addgene

pET51b(+)_CoA-Snifit(V97T)
(Plasmid #197941)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197941 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET51b(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Halo-GFP-ecPanK
  • Species
    other
  • Mutation
    PanK=PanK-V97T, D127A,L277W,F252Y

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET51b(+)_CoA-Snifit(V97T) was a gift from Kai Johnsson (Addgene plasmid # 197941 ; http://n2t.net/addgene:197941 ; RRID:Addgene_197941)

  • For your References section:

    Probing coenzyme A homeostasis with semisynthetic biosensors. Xue L, Schnacke P, Frei MS, Koch B, Hiblot J, Wombacher R, Fabritz S, Johnsson K. Nat Chem Biol. 2022 Oct 31. doi: 10.1038/s41589-022-01172-7. 10.1038/s41589-022-01172-7 PubMed 36316571
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