Skip to main content
Addgene

pEN_TT 3xFLAG-TWIST2
(Plasmid #197935)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEN_TTmiRC2
  • Backbone manufacturer
    Addgene #2752
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TWIST2
  • Alt name
    AMS
  • Alt name
    BBRSAY
  • Species
    H. sapiens (human)
  • Entrez Gene
    TWIST2 (a.k.a. AMS, BBRSAY, DERMO1, FFDD3, SETLSS, bHLHa39)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site MfeI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TT 3xFLAG-TWIST2 was a gift from Kevin Janes (Addgene plasmid # 197935 ; http://n2t.net/addgene:197935 ; RRID:Addgene_197935)

  • For your References section:

    Kinetic networks identify TWIST2 as a key regulatory node in adipogenesis. Dutta AB, Lank DS, Przanowska RK, Przanowski P, Wang L, Nguyen B, Walavalkar NM, Duarte FM, Guertin MJ. Genome Res. 2023 Feb 21;33(3):314-31. doi: 10.1101/gr.277559.122. 10.1101/gr.277559.122 PubMed 36810156
Related items: