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PurposeBL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 197934 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backboneBL21(DE3)
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)B-95.ΔAΔfabR
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Growth instructionsGrowth temperature: 16-37 degrees C
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRNA polymerase gene (T7 phage)/chromosome
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genotype:
The same as BL21(DE3) except for the additional mutations at 95 UAG codons, disruption of prfA, and frameshift in fabR.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
B-95.ΔAΔfabR was a gift from Kensaku Sakamoto (Addgene plasmid # 197934) -
For your References section:
Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon. Mukai T, Hoshi H, Ohtake K, Takahashi M, Yamaguchi A, Hayashi A, Yokoyama S, Sakamoto K. Sci Rep. 2015 May 18;5:9699. doi: 10.1038/srep09699. 10.1038/srep09699 PubMed 25982672