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Addgene

HBT_pcoCASphi_version2
(Plasmid #197932)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HBT
  • Total vector size (bp) 6083
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pcoCasphi-2
  • Alt name
    Arabidopsis codon optimized Casphi-2
  • Alt name
    Cas12j2
  • Species
    Synthetic
  • Promoter 35SPPDK promoter
  • Tag / Fusion Protein
    • 2xNLS-2xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTCACGTAGTAAGCAGCTCTC
  • 3′ sequencing primer CAGAAATTATATGATAATCATCGCAAGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HBT_pcoCASphi_version2 was a gift from Steven Jacobsen (Addgene plasmid # 197932 ; http://n2t.net/addgene:197932 ; RRID:Addgene_197932)

  • For your References section:

    Genome editing in plants using the compact editor CasPhi. Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna JA, Jacobsen SE. Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 10.1073/pnas.2216822120. Epub 2023 Jan 18. 10.1073/pnas.2216822120 PubMed 36652483
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