HBT_pcoCASphi_version1
(Plasmid #197931)

• Purpose: Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal
• Depositing Lab: Steven Jacobsen
• Publication: Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 10.1073/pnas.2216822120. Epub 2023 Jan 18.

Backbone

• Vector backbone: HBT
• Backbone size w/o insert (bp): 3484
• Total vector size (bp): 6110
• Vector type: Plant Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pcoCasphi-2
• Alt name: Arabidopsis codon optimized Casphi-2
• Alt name: Cas12j2
• Species: Synthetic
• Promoter: 35SPPDK promoter
• Tags / Fusion Proteins:
  • FLAG tag and SV40 NLS (N terminal on insert)
  • nucleoplasmin NLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTCACGTAGTAAGCAGCTCTC
• 3′ sequencing primer: CAGAAATTATATGATAATCATCGCAAGAC

Resource Information

• Supplemental Documents:
  • HBT_pcoCASphi_version1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry