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piggybac-EF1-halo-Oct4
(Plasmid #197909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    synthetic
  • Backbone size w/o insert (bp) 7872
  • Total vector size (bp) 9010
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pou5f1
  • Alt name
    pou5f1, oct4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1062
  • GenBank ID
    NM_001252452.1 NP_038661.2
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
  • Promoter EF1
  • Tag / Fusion Protein
    • Halotag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer tttgcctgaccctgcttgc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.10.26.513917v1 for bioRxiv preprint.

Addgene QC found a deletion in EF1a promoter that will not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piggybac-EF1-halo-Oct4 was a gift from James Zhe Liu (Addgene plasmid # 197909 ; http://n2t.net/addgene:197909 ; RRID:Addgene_197909)

  • For your References section:

    Spatial organization of the 3D genome encodes gene co-expression programs in single cells. Dong P, Zhang S, Xie L, Wang L, Lemire AL, Lander AD, Chang HY, Liu ZJ. bioRxiv 2022.10.26.513917 10.1101/2022.10.26.513917
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