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Addgene

pMVQA
(Plasmid #197894)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197894 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR®2.1-TOPO®
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 3929
  • Total vector size (bp) 4541
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 3' end (HXB2: 9045 till poly-A(10)
  • Species
    HIV-1
  • Insert Size (bp)
    612

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer TTTTTTTTTTTTTTTTTTTGAAGCACTC
  • 3′ sequencing primer AGCTGTAGATCTTAGCCACT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMVQA was a gift from Thomas Klimkait (Addgene plasmid # 197894 ; http://n2t.net/addgene:197894 ; RRID:Addgene_197894)

  • For your References section:

    Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periods. Otte F, Zhang Y, Spagnuolo J, Thielen A, Daumer M, Wiethe C, Stoeckle M, Kusejko K, Klein F, Metzner KJ, Klimkait T. Cell Rep Methods. 2023 May 23;3(6):100485. doi: 10.1016/j.crmeth.2023.100485. eCollection 2023 Jun 26. 10.1016/j.crmeth.2023.100485 PubMed 37426753
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