pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)
(Plasmid #197885)

• Purpose: Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre
• Depositing Lab: Kazuto Kobayashi
• Publication: Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):100393. doi: 10.1016/j.crmeth.2022.100393. eCollection 2023 Feb 27.

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Backbone

• Vector backbone: pAAV-MCS
• Total vector size (bp): 7446
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 and DH5alpha are also suitable growth strains.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Species: Aequorea victoria
• Insert Size (bp): 720
• Mutation: N/A
• Promoter: CAG

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tgctaaccatgttcatgc
• 3′ sequencing primer: cagcgtatccacatagcg

Resource Information

• Supplemental Documents:
  • 3.pAAV-CAGGS-Flex_3'USS-GFP(ATG mut).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry