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Addgene

pBBR1.cas9-NT
(Plasmid #197871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Bba_J72114
  • Backbone manufacturer
    Christopher Anderson
  • Backbone size w/o insert (bp) 9334
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    trancCRNA
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    214

Gene/Insert 2

  • Gene/Insert name
    Cas9
  • Alt name
    SpCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4107

Gene/Insert 3

  • Gene/Insert name
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    255

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBBR1.cas9-NT was a gift from Baojun Wang (Addgene plasmid # 197871 ; http://n2t.net/addgene:197871 ; RRID:Addgene_197871)

  • For your References section:

    P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri. Huan YW, Torraca V, Brown R, Fa-Arun J, Miles SL, Oyarzun DA, Mostowy S, Wang B. ACS Synth Biol. 2023 Mar 17;12(3):709-721. doi: 10.1021/acssynbio.2c00465. Epub 2023 Feb 20. 10.1021/acssynbio.2c00465 PubMed 36802585
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