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pTij_sg_mmu_Med6_C_terminal
(Plasmid #197869)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTij_sgRNA_cloning_vector
  • Backbone manufacturer
    self-modified from pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Backbone size w/o insert (bp) 9757
  • Total vector size (bp) 9759
  • Modifications to backbone
    insert 20bp Med6 sgRNA sequence
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Med6
  • Alt name
    Med6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    20
  • Entrez Gene
    Med6 (a.k.a. 1500012F11Rik)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site bbs1 (destroyed during cloning)
  • 3′ cloning site bbs1 (destroyed during cloning)
  • 5′ sequencing primer AGGGTCGGAACAGGAGAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTij_sg_mmu_Med6_C_terminal was a gift from James Zhe Liu (Addgene plasmid # 197869 ; http://n2t.net/addgene:197869 ; RRID:Addgene_197869)

  • For your References section:

    Spatial organization of the 3D genome encodes gene co-expression programs in single cells. Dong P, Zhang S, Xie L, Wang L, Lemire AL, Lander AD, Chang HY, Liu ZJ. bioRxiv 2022.10.26.513917 10.1101/2022.10.26.513917
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