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Addgene

pCMV_PACLight1
(Plasmid #197864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 3986
  • Total vector size (bp) 6176
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PACLight1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2190
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer cgcaaatgggcggtaggcgtg
  • 3′ sequencing primer aacttgtttattgcagct
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.06.579048 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_PACLight1 was a gift from Tommaso Patriarchi (Addgene plasmid # 197864 ; http://n2t.net/addgene:197864 ; RRID:Addgene_197864)

  • For your References section:

    Probing PAC1 receptor activation across species with an engineered sensor. Cola RB, Niethammer SN, Rajamannar P, Gresch A, Bhat MA, Assoumou K, Williams ET, Hauck P, Hartrampf N, Benke D, Stoeber M, Levkowitz G, Melzer S, Patriarchi T. eLife. 2024 Aug 15;13:RP96496. doi: 10.7554/eLife.96496. 10.7554/eLife.96496 PubMed 39145773
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