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pRK5 PSK (745-1235)
(Plasmid #197863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6170
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSK1 (745-1235)
  • Alt name
    PSK1 -alpha (745-1235)
  • Alt name
    TAOK2 (745-1235)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1470
  • Mutation
    The N terminus of PSK1/TAOK2 has been deleted
  • GenBank ID
    9344
  • Entrez Gene
    TAOK2 (a.k.a. MAP3K17, PSK, PSK1, PSK1-BETA, TAO1, TAO2, Tao2beta)
  • Promoter SV40
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer GGTCCAACTGCACCTCGG
  • 3′ sequencing primer GCAATAAACAAGTTGGGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned by Jonathan Morris at KCL (subject to MTA with Addgene)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plese note: This construct originated from a T47-D breast carcinoma cell cDNA library and contains the R1211H variant in PSK1/TAOK2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 PSK (745-1235) was a gift from Jonathan Morris (Addgene plasmid # 197863 ; http://n2t.net/addgene:197863 ; RRID:Addgene_197863)

  • For your References section:

    The prostate-derived sterile 20-like kinase (PSK) regulates microtubule organization and stability. Mitsopoulos C, Zihni C, Garg R, Ridley AJ, Morris JD. J Biol Chem. 2003 May 16;278(20):18085-91. doi: 10.1074/jbc.M213064200. Epub 2003 Mar 13. 10.1074/jbc.M213064200 PubMed 12639963
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