pS548∙CsR
(Plasmid #197859)

• Purpose: Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
• Depositing Lab: Susanne Häußler
• Publication: Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41596-023-00882-z.

Backbone

• Vector backbone: pS448∙CsR
• Backbone size w/o insert (bp): 8398
• Total vector size (bp): 9895
• Vector type: CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA scaffold - Tc resistance cassette - incP oriT
• gRNA/shRNA sequence: gRNA scaffold
• Species: Synthetic
• Insert Size (bp): 1497
• Promoter: PEM7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SanDI (not destroyed)
• 3′ cloning site: FseI (not destroyed)
• 5′ sequencing primer: SEVA_T0-F
• 3′ sequencing primer: PS4

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry