pAd-Klf4
(Plasmid #19770)

• Depositing Lab: Konrad Hochedlinger
• Publication: Stadtfeld et al Science. 2008 Sep 25. ():.

Backbone

• Vector backbone: pAd-ClaI
• Backbone size w/o insert (bp): 32900
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Klf4
• Species: M. musculus (mouse)
• Insert Size (bp): 3800
• Entrez Gene: Klf4 (a.k.a. EZF, Gklf, Zie)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MfeI (unknown if destroyed)
• 3′ cloning site: PacI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest- SpeI
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.