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vYM141_phiC31_nTRE-pEF1s(orig)(CC793)-H2B-citrine
(Plasmid #197684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PhiC31
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 6200
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pEF1s-H2B-Citrine
  • Species
    Synthetic
  • Insert Size (bp)
    1900

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

R129P mutation in the H2B-citrine ORF will not affect plasmid function. Please visit https://doi.org/10.1101/2023.05.30.542205 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vYM141_phiC31_nTRE-pEF1s(orig)(CC793)-H2B-citrine was a gift from Michael Elowitz (Addgene plasmid # 197684 ; http://n2t.net/addgene:197684 ; RRID:Addgene_197684)

  • For your References section:

    Tuning Methylation-Dependent Silencing Dynamics by Synthetic Modulation of CpG Density. Ma Y, Budde MW, Zhu J, Elowitz MB. ACS Synth Biol. 2023 Aug 12. doi: 10.1021/acssynbio.3c00078. 10.1021/acssynbio.3c00078 PubMed 37572041
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