pAd-Oct4
(Plasmid #19768)

• Depositing Lab: Konrad Hochedlinger
• Publication: Stadtfeld et al Science. 2008 Sep 25. ():.

Backbone

• Vector backbone: pAd-ClaI
• Backbone size w/o insert (bp): 32900
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Oct4
• Species: M. musculus (mouse)
• Insert Size (bp): 2200
• Entrez Gene: Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MfeI (unknown if destroyed)
• 3′ cloning site: PacI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.