4A3.S'U'
(Plasmid
#197676)
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PurposeExpresses P1 S' tail fibre and U' chaperone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSC101 (4A3)
- Backbone size w/o insert (bp) 3343
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameS'
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SpeciesBacteriophage P1 (E. coli P1 lysogen EMG16)
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Insert Size (bp)2919
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GenBank IDN/A N/A
- Promoter Late S promoter, LPS
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer gaggcttcacaacattgattag
- 3′ sequencing primer ttttgttttagggttgccag (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameU'
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SpeciesBacteriophage P1 (E. coli P1 lysogen EMG16)
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Insert Size (bp)532
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GenBank IDN/A N/A
- Promoter N/A
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer gcattaatgggacgtggtataac
- 3′ sequencing primer ctcgagtgcggccgcaagcttgtcg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4A3.S'U' was a gift from Baojun Wang (Addgene plasmid # 197676 ; http://n2t.net/addgene:197676 ; RRID:Addgene_197676) -
For your References section:
The Role of O-antigen in P1 Transduction of Shigella flexneri and Escherichia coli with its Alternative S' Tail Fibre. Huan YW, Fa-Arun J, Wang B. J Mol Biol. 2022 Sep 15;434(21):167829. doi: 10.1016/j.jmb.2022.167829. 10.1016/j.jmb.2022.167829 PubMed 36116540