pCDF_HA_CASP_sigma
(Plasmid
#197614)
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PurposeFor expression of HA-tagged CASP sigma in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDF
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHA-tagged CASP sigma
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF_HA_CASP_sigma was a gift from Feng Zhang (Addgene plasmid # 197614 ; http://n2t.net/addgene:197614 ; RRID:Addgene_197614) -
For your References section:
RNA-activated protein cleavage with a CRISPR-associated endopeptidase. Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F. Science. 2022 Nov 25;378(6622):874-881. doi: 10.1126/science.add7450. Epub 2022 Nov 3. 10.1126/science.add7450 PubMed 36423276