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pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]
(Plasmid #197599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    Joachim Messing
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 5000
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synaptogyrin-CRY2 (D387A+E490G + residues 1-535)
  • Alt name
    SNG-1::CRY2(D387A)olig(535)
  • Alt name
    SNG-1
  • Alt name
    CRY2
  • Species
    C. elegans (nematode), A. thaliana (mustard weed)
  • Insert Size (bp)
    3483
  • Mutation
    D387A, E490G
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Entrez Gene
    sng-1 (a.k.a. CELE_T08A9.3)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry] was a gift from Alexander Gottschalk (Addgene plasmid # 197599 ; http://n2t.net/addgene:197599 ; RRID:Addgene_197599)

  • For your References section:

    Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles. Vettkotter D, Schneider M, Goulden BD, Dill H, Liewald J, Zeiler S, Guldan J, Ates YA, Watanabe S, Gottschalk A. Nat Commun. 2022 Dec 19;13(1):7827. doi: 10.1038/s41467-022-35324-z. 10.1038/s41467-022-35324-z PubMed 36535932
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