pDV18 [punc-47::SNG-1::CRY2olig(535)]
(Plasmid
#197598)
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PurposeExpression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegans
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCFJ201
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynaptogyrin-CRY2 (E490G + residues 1-535)
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Alt nameSNG-1::CRY2olig(535)
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Alt nameSNG-1
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Alt nameCRY2
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SpeciesC. elegans (nematode), A. thaliana (mustard weed)
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Insert Size (bp)3483
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MutationE490G
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
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Entrez Genesng-1 (a.k.a. CELE_T08A9.3)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDV18 [punc-47::SNG-1::CRY2olig(535)] was a gift from Alexander Gottschalk (Addgene plasmid # 197598 ; http://n2t.net/addgene:197598 ; RRID:Addgene_197598) -
For your References section:
Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles. Vettkotter D, Schneider M, Goulden BD, Dill H, Liewald J, Zeiler S, Guldan J, Ates YA, Watanabe S, Gottschalk A. Nat Commun. 2022 Dec 19;13(1):7827. doi: 10.1038/s41467-022-35324-z. 10.1038/s41467-022-35324-z PubMed 36535932