Skip to main content
Addgene

pLenti-hSyn1-mRuby2-T2A-GCaMP6f
(Plasmid #197595)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 8431
  • Total vector size (bp) 10558
  • Modifications to backbone
    hSynI promoter
  • Vector type
    Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRuby2-T2A-GCaMP6f
  • Insert Size (bp)
    2127
  • Promoter hSynI

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer agtcgtgtcgtgcctgag
  • 3′ sequencing primer TGTTGCTCCTTTTACGCTATG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    From Addgene's plasmid #50943

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-hSyn1-mRuby2-T2A-GCaMP6f was a gift from Patrik Verstreken (Addgene plasmid # 197595 ; http://n2t.net/addgene:197595 ; RRID:Addgene_197595)
Related items:
Commonly requested with: