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Addgene

pCMVlux-cp157Venus
(Plasmid #197584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human codon-optimized Lux-cp157Venus
  • Species
    Synthetic
  • Promoter CMV promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAATACCATATGGATCTCATGCAGAAGAACGGCATC
  • 3′ sequencing primer TGATCCCCCACCGATCTTGTCGGCGGTGATATAGAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    490 Biotech

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVlux-cp157Venus was a gift from Takeharu Nagai (Addgene plasmid # 197584 ; http://n2t.net/addgene:197584 ; RRID:Addgene_197584)

  • For your References section:

    Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer. Kaku T, Sugiura K, Entani T, Osabe K, Nagai T. Sci Rep. 2021 Jul 22;11(1):14994. doi: 10.1038/s41598-021-94551-4. 10.1038/s41598-021-94551-4 PubMed 34294849
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