luxB:cp157Venus+luxA/pRSETB Duet
(Plasmid #197583)

• Purpose: BRET lux luciferase with cp157Venus for bacterial expression
• Depositing Lab: Takeharu Nagai
• Publication: Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 10.1038/s41598-021-94551-4.

Backbone

• Vector backbone: pRSETB
• Backbone manufacturer: invitrogen
• Vector type: Bacterial Expression ; E.coli expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LuxA LuxBcp157Venus
• Species: Synthetic
• Insert Size (bp): 2784
• GenBank ID:
• Promoter: T7
• Tag / Fusion Protein:
  • His X 6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CACCGAGCTCATGCAGAAGAACGGCATCAAG
• 3′ sequencing primer: CATCGCGGCCGCTTACTTGTCGGCGGTGATA

Resource Information

• Supplemental Documents:
  • LuxBcp157Venus luxA.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry