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Addgene

luxA+luxB/pRSETB Duet
(Plasmid #197581)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197581 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETb
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LuxA LuxB
  • Species
    Synthetic
  • Insert Size (bp)
    2067
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • T7 tag and 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer CACCGGATCCGATGAAATTTGGAAACTTTTTG
  • 3′ sequencing primer CACCGGTACCCTAATATAATAGCGAACGTTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    luxA+luxB/pRSETB Duet was a gift from Takeharu Nagai (Addgene plasmid # 197581 ; http://n2t.net/addgene:197581 ; RRID:Addgene_197581)

  • For your References section:

    Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer. Kaku T, Sugiura K, Entani T, Osabe K, Nagai T. Sci Rep. 2021 Jul 22;11(1):14994. doi: 10.1038/s41598-021-94551-4. 10.1038/s41598-021-94551-4 PubMed 34294849
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