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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19757 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneEGFP-N1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOrai1
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Alt nameORAI calcium release-activated calcium modulator 1
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SpeciesH. sapiens (human)
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Entrez GeneORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
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Tag
/ Fusion Protein
- CFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Orai1 CFP was a gift from Anjana Rao (Addgene plasmid # 19757 ; http://n2t.net/addgene:19757 ; RRID:Addgene_19757) -
For your References section:
Orai1 is an essential pore subunit of the CRAC channel. Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG. Nature. 2006 Sep 14. 443(7108):230-3. 10.1038/nature05122 PubMed 16921383