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pAcBac1-Ma-sfGFP 150TAG
(Plasmid #197568)

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Item Catalog # Description Quantity Price (USD)
Plasmid 197568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcBac1
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 9795
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sfGFP 150TAG
  • Insert Size (bp)
    723
  • Mutation
    N150TAG
  • Promoter CMV
  • Tag / Fusion Protein
    • V5-His6 (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer cgcaaatgggcggtaggcgtg
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    M. alvus Pyl-tRNA (6) (4x copies)
  • Species
    Methanomethylophilus alvus
  • Insert Size (bp)
    69
  • Promoter U6/H1

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid should be paired with another pAcBac1 vector that expresses both a M. alvus PylRS variant/M. alvus Pyl-tRNA pair

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac1-Ma-sfGFP 150TAG was a gift from Ryan Mehl (Addgene plasmid # 197568 ; http://n2t.net/addgene:197568 ; RRID:Addgene_197568)

  • For your References section:

    Generating Efficient Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetases for Structurally Diverse Non-Canonical Amino Acids. Avila-Crump S, Hemshorn ML, Jones CM, Mbengi L, Meyer K, Griffis JA, Jana S, Petrina GE, Pagar VV, Karplus PA, Petersson EJ, Perona JJ, Mehl RA, Cooley RB. ACS Chem Biol. 2022 Dec 16;17(12):3458-3469. doi: 10.1021/acschembio.2c00639. Epub 2022 Nov 16. 10.1021/acschembio.2c00639 PubMed 36383641
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